Turkish Journal of Anaesthesiology & Reanimation
ORIGINAL ARTICLE

Protective Role of Dexmedetomidine on Ileum and Kidney Damage Caused by Mesenchymal Ischaemia in Rats

1.

Department of Anaesthesiology and Reanimation, Yavuz Selim Bone Diseases and Rehabilitation Hospital, Trabzon, Turkey

2.

Department of Anaesthesiology and Reanimation, University of Health Sciences Ordu University Training and Research Hospital, Ordu, Turkey

3.

Department of Anaesthesiology and Reanimation, Karadeniz Technical University, Trabzon, Turkey

Turk J Anaesthesiol Reanim 2018; 46: 470-477
DOI: 10.5152/TJAR.2018.46244
Read: 433 Downloads: 225 Published: 21 September 2019

Objective: The aim of this study was to demonstrate ischaemia reperfusion (IR) injury on the ileum and kidney tissue in rats and to evaluate the effect of dexmedetomidine administered at different doses and dosing schedules on recovery.

Methods: A total of 30 rats were randomly divided into five groups. Group I: sham; Group II: control; Group III: dexmedetomidine before ischaemia; Group IV: dexmedetomidine after ischaemia; and Group V: dexmedetomidine before and after ischaemia. The malondialdehyde (MDA) and signal peptide-CUBEGF (epidermal growth factor) domain-containing protein 1 (SCUBE-1) levels of all subjects were studied from the serum, ileum, and kidney tissues. Moreover, the histopathology of ileum and kidney tissues was examined.

Results: The SCUBE-1 levels were found to be highly similar to the MDA levels in ischaemic groups. The serum SCUBE-1 levels obtained were significantly lower in Group V compared to Groups II, III and IV (p<0.001, p=0.003, p=0.013, respectively). The apoptosis indexes were found to be lower in groups receiving dexmedetomidine compared to Group II. The groups receiving dexmedetomidine were detected to have normal morphological appearance when compared to Group II.

Conclusion: In this study, the use of dexmedetomidine in the preoperative and peroperative periods may be beneficial in reducing the negative effects of IR injury.

 

Cite this article as: Sancaktar NÇ, Altınbaş A, Çekiç B. Protective Role of Dexmedetomidine on Ileum and Kidney Damage Caused by Mesenchymal Ischaemia in Rats. Turk J Anaesthesiol Reanim 2018; 46(6): 470-7.

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