Turkish Journal of Anaesthesiology & Reanimation
Paediatric Anaesthesia - ORIGINAL ARTICLE

Effect of Isoflurane Exposure with Administration of Polyunsaturated Fatty Acids on Cognition in Developing Rats


Department of Anaesthesiology and Reanimation, Health Ministry, Health Sciences University, Bağcılar Training and Research Hospital, İstanbul, Turkey

Turk J Anaesthesiol Reanim 2020; 48: 477-483
DOI: 10.5152/TJAR.2020.128
Read: 326 Downloads: 181 Published: 20 November 2020

Objective: The developing brain is vulnerable to the negative effects of anaesthetics. We aimed to investigate the effect of isoflurane and polyunsaturated fatty acids (PUFAs) on cognition.

Methods: A total of 64, ten days old rats were randomly divided into 4 groups: group O2 (oxygen group), group Iso (isoflurane group), group Iso-S (isoflurane+saline) and group Iso-PUFAs (isoflurane+intraperitoneal [IP] PUFAs emulsion). Rats in groups Iso, Iso-S and Iso-PUFAs were exposed to 1.5% isoflurane in 50% oxygen for 6 hours. Rats in group O2 breathed only 50% oxygen. Before anaesthesia, rats in group Iso-S were administered 0.5 mL isotonic and rats in group Iso-PUFAs were administered 5 mL kg-1 PUFAs emulsion by IP injection. The Morris water maze (MWM) test was performed on postnatal 28-33 days. Histological evaluation and immune histochemical staining (Bcl-2 antibody) were performed on postnatal day 11 on rat brains.

Results: As demonstrated by the reduction in the escape latency on days 3, 4 and 5 compared with day 1, all rats learned the task during the acquisition period. In contrast to others, rats in group Iso spent significantly lower time to find the platform on day 2 than on day 1 (p=0.034). No significant difference was found among the groups in terms of time spent in finding the platform. There were no significant differences in probe trials, histological features and Bcl-2 immunoreactivity among the groups.

Conclusion: Isoflurane did not cause cognitive dysfunction and neuronal death, and a single dose of PUFAs emulsion had no effect on cognition either.

Cite this article as: Aldemir Şensoy D, Demirgan S, Akyol O, Gümüş Özcan F, Selcan A. Effect of Isoflurane Exposure with Administration of Polyunsaturated Fatty Acids on Cognition in Developing Rats. Turk J Anaesthesiol Reanim 2020; 48(6): 477-83.

EISSN 2667-6370